Enzymatic hydrolysis of defatted mackerel protein with low bitter taste     

Hu Hou  Bafang Li  Xue Zhao 《中国海洋大学学报(英文版)》2011,10(1):85-92

Ultrasound-assisted solvent extraction was confirmed as a novel,effective method for separating lipid from mackerel pro-tein,resulting in a degreasing rate (DR) of 95% and a nitrogen recovery (NR) of 88.6%.To obtain protein hydrolysates with high ni-trogen recovery and low bitter taste,enzymatic hydrolysis was performed using eight commercially available proteases.It turned out that the optimum enzyme was the ‘Mixed enzymes for animal proteolysis’.An enzyme dosage of 4%,a temperature of 50℃,and a hydrolysis time of 300 min were found to be the optimum conditions to obtain high NR (84.28%) and degree of hydrolysis (DH,16.18%) by orthogonal experiments.Glutamic acid was the most abundant amino acid of MDP (defatted mackerel protein) and MDPH (defatted mackerel protein hydrolysates).Compared with the FAO/WHO reference protein,the essential amino acid chemical scores (CS) were greater than 1.0 (1.0 1.7) in MDPH,which is reflective of high nutritional value.This,coupled with the light color and slight fishy odor,indicates that MDPH would potentially have a wide range of applications such as nutritional additives,functional ingredients,and so on.  相似文献   

珠江三角洲滨海小流域离子化学特征及来源分析   总被引：1，自引：0，他引：1  

谢丽纯  陈建耀  董林垚  蒋华波 《热带地理》2011,31(2)

在分析中山大学滨海小流域水循环综合试验基地内雨水、河水和地下水离子组分的基础上,讨论了该小流域内水体离子化学组分的特征及来源。结果显示:①河水中TDS变化范围为28.97～44.64 mg/l,平均值为33.90 mg/l;在离子化学组成中,阳离子以Na++K+为主,阴离子以Cl-和HCO3-为主;雨水中TDS的变化范围为0.88～26.07 mg/l,平均值为8.42 mg/l,离子含量变化幅度较大;地下水TDS变化范围为39.49～113.16 mg/l,均值为58.11 mg/l,约为流域河水的2倍,雨水的7倍;②海盐沉降中Ca2+、Mg2+、Na+、K+、SO42-的贡献率:雨季分别为6%、60%、47%、2%和15%,旱季分别为3%、41%、54%、2%、9%;③硅酸盐风化中,雨季:(Ca/Na)sil=0.31,(Mg/K)sil=0.21;旱季:(Ca/Na)sil=0.36,(Mg/K)sil=0.27;CO2消耗率为12.4 t/(km2.a);④河水中的NO3-和(SO42-)res主要来源于大气酸沉降,且具有旱季含量低、雨季含量高的特点;⑤R1潜水井受到一定程度的NO3-污染,NO3-的...  相似文献   

不同温度、羧酸溶液中长石溶解模拟实验   总被引：24，自引：2，他引：22  

向廷生  蔡春芳  付华娥 《沉积学报》2004,22(4):597-602

报道了在100℃、140℃下微斜长石在不同羧酸溶液中的溶解实验数据。通过实验表明1)反应温度增高,可增强溶液中阳离子的活性和迁移性,加快长石溶解的反应速率,促进长石的溶解。2)在强酸性条件下,pH值的变化可影响长石的溶解。但在中等酸性条件下,pH值对长石的溶解影响很小。3)羧酸(乙二酸)可不同程度地促进长石溶解,可通过形成乙二酸络合物的形式,增加离子在溶液中的溶解度。但乙酸络合物的作用不明显。长石溶蚀导致岩石孔隙度变大,并且改善孔喉性质。同时,由于乙二酸络合物的存在,增加了Si在溶液中的溶解度,阻止了石英加大和其它成因SiO₂的生成,有利于次生孔隙和原生孔隙的保存。4)长石溶解使溶液中Al的浓度较高,但由于铝-羧酸络合物的亲油性比亲水性强,故有一部分Al被分配到油相中,这也是目前大多数油田水中Al浓度偏低的主要原因。  相似文献   

金川超大型铜镍硫化物矿床的铂族元素地球化学特征   总被引：21，自引：2，他引：19  

王瑞廷  毛景文  赫英  王东生  汤中立 《大地构造与成矿学》2004,28(3):279-286

对金川超大型铜镍岩浆硫化物矿床岩石、矿石的铂族元素地球化学特征研究表明 ,金川岩体的平均Cu/Pd值远大于原生地幔岩浆的Cu/Pd值 ,说明其岩石为因硫化物析离而失去Pd的岩浆所结晶 ;且岩石的PGE具有部分熔融趋势 ,与地幔橄榄岩接近 ,这些均指示存在岩浆熔离作用。该矿床岩石、矿石的PGE球粒陨石标准化分布模式比较对应 ,均可分为两种类型 ,反映了岩浆多次侵入、熔离分异同时成岩成矿的特征。另外 ,PGE S关系分析表明其成岩成矿过程中有少量地壳物质混染。PGE地球化学特征参数还指示了其高镁拉斑玄武质母岩浆的性质。  相似文献   

河嘉203H含硫水平气井测试工艺实践     

唐波  乔智国  陈伟  叶翠莲 《海洋地质译丛》2010,(4):103-105

河嘉203H井是川东北河坝构造嘉二高压含硫气藏部署的第一口水平井,具有埋藏深、储层压力高、腐蚀分压高、地层易漏失、水平井压井堵漏难度大的特点,通过开展工具优选、管柱结构优化设计、流程优选,并对替浆、放喷油嘴控制及压井等主要工序的施工工艺参数进行研究,形成了河坝嘉二含硫气藏水平井测试工艺技术体系,并在河嘉203H井获得成功应用。  相似文献   

Aminostratigraphy and sea-level history of the Pleistocene Bridgewater Formation,Mount Gambier region,southern Australia     

A. G. Blakemore  K. E. Westaway  T. J. Lachlan 《Australian Journal of Earth Sciences》2013,60(2):151-169

A geochronological framework based on amino acid racemisation (AAR) and constrained by previously reported optically stimulated luminescence (OSL) ages is presented for the evolution and paleosea-level record of the Pleistocene Bridgewater Formation of the Mount Gambier region, of southern Australia. Within the study area, the Bridgewater Formation is represented by late early Pleistocene [Marine Isotope Stage (MIS) 23 at 933 ka] to Holocene barrier shoreline successions deposited during sea-level highstands. Regional monotonic uplift (0.13 mm yr^–1) and pervasive calcrete development during the Pleistocene have preserved the sequence of calcarenite (mixed quartz-skeletal carbonate sand) shoreline complexes from denudation. AAR analyses confirm that the barriers generally increase in age landwards and correlate with sea-level highstands associated with interglacials as defined by the marine oxygen isotope record. AAR analyses on the benthic foraminifer Elphidium crispum have proved more reliable than the whole-rock method in extending the age range of AAR dating of these relict shoreline successions. Paleosea-levels from the coastal plain are as follows: MIS 7, –9 ± 2 m; MIS 9, 4 ± 1 m; and a minimum sea-level of 2 ± 2 m is derived for MIS 11. Paleosea-level could not be determined for MIS 15, 19 or 23 as diagnostic sea-level indicators were not identified within these sedimentary successions. Dismal Range, dated at 933 ± 145 ka (MIS 23), represents a correlative feature to the East Naracoorte Range but is some 25 km seaward of the Kanawinka Fault compared with the same barrier at Naracoorte. Mingbool Range (788 ± 18 ka) is of similar age to the West Naracoorte Range (MIS 19) and formed as an arcuate shoreline complex that became attached to the higher relief of the area represented by the Mount Burr Volcanic Province. The higher topographical relief resulted from crustal doming of the Oligo-Miocene Gambier Limestone caused by the intrusion of magma associated with the volcanic province. The AAR age of 788 ± 118 ka for Mingbool Range indicates that the Mount Burr volcanics predate the deposition of this shoreline complex.  相似文献   

Improved age constraint for pre‐ and post‐Anglian temperate‐stage deposits in north Norfolk,UK, from analysis of serine decomposition in Bithynia opercula     

Rob Westaway 《第四纪科学杂志》2010,25(5):715-723

The ‘new glacial stratigraphy’ (NGS) of Britain postulates that deposits hitherto assigned to the Anglian glaciation in Marine Isotope Stage (MIS) 12 represent MIS 16, 12, 10 and 6. This controversial idea can be tested at Sidestrand in north Norfolk, where fossiliferous temperate‐stage deposits underlie the oldest and overlie the youngest of the glacial deposits. Previous work has considered biostratigraphy and amino acid dating of these temperate‐stage deposits, but did not achieve tight age constraint using the amino acid evidence alone. The total hydrolysable amino acid fraction in intra‐crystalline protein from Bithynia tentaculata opercula has previously been analysed for concentrations of serine and alanine. Statistical screening of these data gives alanine/serine ratios of 4.572 ± 0.114 for the Sidestrand Hall Member (beneath the glacial deposits) and 3.564 ± 0.091 for the Sidestrand Cliff Formation (overlying the glacial deposits). These ratios imply ages of MIS 13a and 11c, respectively, indicating the latest Cromerian and early Hoxnian interglacials and invalidating the NGS. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

Spatial patterns of groundwater‐lake exchange – implications for acid neutralization processes in an acid mine lake     

Christiane Neumann  Julia Beer  Christian Blodau  Stefan Peiffer  Jan H. Fleckenstein 《水文研究》2013,27(22):3240-3253

Exchange of groundwater and lake water with typically quite different chemical composition is an important driver for biogeochemical processes at the groundwater‐lake interface, which can affect the water quality of lakes. This is of particular relevance in mine lakes where anoxic and slightly acidic groundwater mixes with oxic and acidic lake water (pH < 3). To identify links between groundwater‐lake exchange rates and acid neutralization processes in the sediments, exchange rates were quantified and related to pore‐water pH, sulfate and iron concentrations as well as sulfate reduction rates within the sediment. Seepage rates measured with seepage meters (?2.5 to 5.8 L m^‐2 d^‐1) were in reasonable agreement with rates inverted from modeled chloride profiles (?1.8 to 8.1 L m^‐2 d^‐1). Large‐scale exchange patterns were defined by the (hydro)geologic setting but superimposed by smaller scale variations caused by variability in sediment texture. Sites characterized by groundwater upwelling (flow into the lake) and sites where flow alternated between upwelling and downwelling were identified. Observed chloride profiles at the alternating sites reflected the transient flow regime. Seepage direction, as well as seepage rate, were found to influence pH, sulfate and iron profiles and the associated sulfate reduction rates. Under alternating conditions proton‐consuming processes, for example, sulfate reduction, were slowed. In the uppermost layer of the sediment (max. 5 cm), sulfate reduction rates were significantly higher at upwelling (>330 nmol g^‐1 d^‐1) compared to alternating sites (<220 nmol g^‐1 d^‐1). Although differences in sulfate reduction rates could not be explained solely by different flux rates, they were clearly related to the prevailing groundwater‐lake exchange patterns and the associated pH conditions. Our findings strongly suggest that groundwater‐lake exchange has significant effects on the biogeochemical processes that are coupled to sulfate reduction such as acidity retention and precipitation of iron sulfides. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

海洋产油真菌的简便初筛   总被引：1，自引：0，他引：1  

秦艳红  叶德赞 《台湾海峡》2010,29(1):128-134

选取分离自大洋、深海的28株酵母和143株霉菌，采用在培养基中添加尼罗红比较表观荧光强度的方法来筛选出酵母2株和霉菌7株，但菌体呈红色的酵母（17株）及菌丝为黑色的霉菌（28株）不适用于此法；通过单位DD鲫荧光强度的高低筛选出1株菌落为微红色的酵母，产油性能好于用第一种方法挑选出的2株酵母，且菌体颜色对结果并无影响，但只适合筛选酵母．通过上述2种方法筛选出高油脂菌株，大多来源于深海．  相似文献   

王588块泥浆污染深穿透酸液研究与评价     

胡增国 《海洋地质译丛》2010,(2):58-62,71

王588块钻井泥浆污染主要是固相入侵和滤液与地层水的不配伍性。通过实验,优选出了具有高效深穿透能力以及固相溶解量大的表面活性剂DS-101酸液复配体系。实验表明,该体系耐温性强,对油泥具有极强的分散和溶解作用,对泥浆污染有明显的解堵效果,岩心渗透率恢复率可达127%。  相似文献